Comparative Analysis of Expression of the Main Virulence Genes in Various <i>Vibrio cholerae</i> О1 Strains

The aim of the work was a comparative study expression main virulence genes in Vibrio cholerae strains classical biovar, typical and genetically modified V. , El Tor biovar. Materials methods . Natural toxigenic O1, biovar (J89, Pakistan, 1969), (M-887, Astrakhan, 1970) (301, Taganrog, 2011) were used as model ones. grown under optimum conditions for production cholera toxin toxin-coregulated pili. assessment strain growth carried out LB broth at room temperature with determination cells number on Biowave DNA spectrophotometer (Biochrome Ltd., UK). Determination gene performed using real-time PCR reverse transcription. Results discussion structural ( ctxA tcpA ) regulatory toxR toxT tcpP tcpH has been investigated Significant differences have revealed terms time level maximum these biovars. It found that genovariant reached its 1–3 h earlier than other strains. At same time, corresponded to strain. higher strains, also had clear inverse correlation expression. Expression 1–2 These should be taken into account when conducting research related genes.